TurboDoma® media for hybridomas

Our TurboDoma nutrient mixtures comprise chemically defined, protein and peptide-free minimal culture media for the cultivation of several hybridomas and myeloma cell lines, including the authenticated and certified serum-free cell lines Sp2/0-TurboDoma, P3X63Ag8.653-TurboDoma and NS0-TurboDoma available at the European Collection of Animal Cell Cultures.

For more details please also refer to StarterKits.


TurboDoma Media display the following features:

  • Exclusively made of small molecules characterised by their CAS- and EINECS registry numbers

  • Totally free of proteins, peptides, complex additives such as albumines, hydrolysates and yeast extracts, and animal-derived components


    TurboDoma medium, kit for the preparation of 20 liters medium.
    The complete kit consists of 5 bottles.

    We currently offer the following TurboDoma media formulations:

  • TurboDoma, art. THP, medium for the routine maintenance and the cryopreservation

  • TurboDoma TP-6, art. TTP6, medium for mass cell cultivation and production purposes


    The TurboDoma medium, art. THP, is a minimal nutrient mixture for the protein and peptide-free selection, routine maintenance and cryopreservation of cholesterol-independent myeloma and hybridoma cells, including the Sp2/0-TurboDoma (ECACC accession no. 03092501), P3X63Ag8.653-TurboDoma (ECACC accession no. 03092502), and NS0-TurboDoma (ECACC accession no. 06020202) cell lines. The medium can be used to routinely grow cells in static or agitated culture systems such as tissue culture flasks, spinner flasks, shaker flasks, roller bottles, membrane reactors (i.e. Integra’s CELLine), stirred fermentors, and hollow fiber reactors. Click here to download the protocol for the serum-free generation of hybridomas with our TurboDoma medium.

    The TurboDoma TP-6 medium, art. TTP6, is a fortified nutrient mixture for the production of monoclonal antibodies and recombinant proteins with hybridomas and myeloma cells. TurboDoma TP-6 is typically used for batch, fed batch, and perfusion culture processes. According to experience accumulated over almost two decades, TurboDoma TP-6 medium often outperforms commercially available competitors’ media in terms of both cell density and productivity when growing hybridomas generated with myeloma fusion partners such as Sp2/0-Ag14, P3UI, and P3X63Ag8.653.

    TurboDoma media are available as 1X liquid preparations ready-for-use as well as kits of highly concentrated liquid solutions for the reconstitution of 8, 20, 200, and 2000 liters 1X medium.
    The liquid concentrates have to be reconstituted with tissue culture water prior to sterile filtration according to the method of preparation enclosed in the product package. The method of preparation of the TurboDoma media can de downloaded by clicking on the link below.

  • Procedure for the preparation of 8 liters medium
  • Procedure for the preparation of 20 liters medium
  • Procedure for the preparation of 200 liters medium

    TurboDoma® is a registered trademark of Dr. F. Messi