Hektor™ media for 293 cells

The chemically defined protein- and peptide-free Hektor media were originally developed for the cultivation of kidney-derived human cell lines and the expression of recombinant glycoproteins in HEK 293, HEK 293T and HEK 293EBNA cells. Hektor media have also been used for the adaptation of serum-dependent Vero cells to serum-free culture conditions.

The authenticated and certified serum-free 293-Hektor cell line (ECACC accession no. 05030204) and Vero-Hektor cell line (ECACC accession no. 03092503) available at the European Collection of Animal Cell Cultures are routinely maintained and banked with Hektor G medium.

For more details please refer to StarterKits.


Hektor media display the following features:

  • Exclusively made of small molecules characterised by their CAS- and EINECS registry numbers

  • Totally free of proteins, peptides, complex additives such as albumines, hydrolysates and yeast extracts, and animal-derived components

    Hektor G medium, kit for the preparation of 8 liters medium.
    The complete kit consists of 5 bottles.

    We offer the following Hektor nutrient mixtures:

  • Hektor G, art. HEKG, medium for routine maintenance and cryopreservation

  • Hektor S, art. HEKS, medium for bioprocess applications

    Hektor G-adapted 293 cells can be easily transferred to the nutrient-enriched Hektor S medium for bioprocess development and production.
    The Hektor S medium, art. HEKS, is designed for the chemically defined production of recombinant proteins and viral particles with HEK 293, HEK 293EBNA and HEK 293T cells.
    Among the main advantages, the total absence of large molecules from Hektor media facilitates the purification of retroviral particles for cell and gene therapy applications.

    Hektor media are available as 1X liquid preparations ready-for-use as well as kits of highly concentrated liquid solutions for the reconstitution of 8, 20 and 200 liters 1X medium.
    The liquid concentrates are reconstituted with tissue culture water prior to sterile filtration.
    Click on the links below to see the method of medium reconstitution.

  • Procedure for the preparation of 8 liters medium
  • Procedure for the preparation of 20 liters medium
  • Procedure for the preparation of 200 liters medium

    Hektor™ is a trademark of Dr. F. Messi Cell Culture Technologies.