ChoMaster®EPO is provided as open-source technology package featuring culture media and simple working protocols to get acquainted with the minimal maintenance technique.
ChoMaster®EPO is part of Cell Culture Technologies’ ChoMaster® Biosimilar Series.
The 22-years-old history of the host cell line used to generate ChoMaster®EPO cells has been recorded since the very first days of cultivation in Cell Culture Technologies’ minimal media. A wide range of recombinant proteins have been successfully expressed with our CHO-K1 cells adapted to our minimal nutrient mixtures since 1993.
Several academic institutions, small biotechs and big pharma companies licensed our unique know-how to implement and develop tailor-made bioprocess solutions. ChoMaster®EPO cells were genetically engineered by Jack Rohrer, Bettina Keller Abu Seda and Jenny Pally-Eggenschwiler, Institute of Cell Biology, Zurich University of Applied Sciences, Waedenswil, Switzerland.
ChoMaster® minimal media are exclusively made of small molecules of highest purity characterised by their respective CAS/EINECS numbers. ChoMaster® HP media are fortified nutrient mixtures that contain no ingredients of human or animal origin, no recombinant proteins, peptides, peptones or yeast extracts, and no hydrolysates. A proprietary manufacturing technique coupled with the detailed estimate of the nutritional requirements of ChoMaster®EPO cells have led to the development of several ChoMaster® media formulations to fit several possible culture processes, from single cell cloning (ChoMaster® HTS) over routine maintenance (ChoMaster® HP-1) to high density perfusion culture (ChoMaster® HP-5).
FMX-8 medium, Ferruccio Messi’s medium X version no. 8, is a chemically defined minimal nutrient mixture for CHO cells made of 43 small molecules. The complete formulation of the FMX-8 medium with the identity and the concentration of every ingredient was published in 1991 and 1993 by Ferruccio Messi, and 1995 by Michael Zang, Helmut Trautmann, Christine Gandor, Ferruccio Messi, Fred Asselbergs, Christian Leist, Armin Fiechter and Jakob Reiser.
ChoMaster®EPO cells easily proliferate in the protein- and peptide-free ChoMaster® and FMX-8 minimal culture media developed and manufactured by Cell Culture Technologies. While full reproducibility of the media composition allows wide control over cell proliferation and recombinant protein expression, ChoMaster and FMX-8 media can be obtained under a toolbox format to investigate the characteristics of the secreted glycoprotein of interest in great details. Our Media Toolbox Kits consist of a basal medium combined with a set of seven concentrated solutions for investigational purposes. For example, the Titer Enhancer Toolbox consists of a glucose-free basal medium – either ChoMaster or FMX-8 – and seven concentrated solutions with amino acids formulated into metabolically related groups for the purpose of increasing cellular productivity. When properly combined, the components of the Media Toolbox Kits may generate new glycoproteins (isoforms) of interest.
Please find a selection of available ChoMaster & FMX-8 Media Toolbox Kits below:
Technical support regarding freezing, thawing and maintaining ChoMaster®EPO cells in ChoMaster® and FMX-8 media is provided together with the media and the cells, so that recombinant EPO expression can be immediately accomplished.
ChoMaster® is a registered trademarks of Dr. F. Messi Cell Culture Technologies.