Procedure for the preparation of 200 liters medium
Culture media Hektor, InVitrus, TurboDoma
Fortified culture media InVitrus VP-6, TurboDoma TP-6
Hektor, InVitrus & TurboDoma media kits consist of 5 vials containing liquid solutions.
InVitrus VP-6 & TurboDoma TP-6 media kits consist of 6 vials containing liquid solutions.
Sodium hydrogen carbonate and L-glutamine have to be added separately.
Store the kit at -20ºC for optimal preservation.
After thawing, shake the vials vigorously before use!
Time required for preparing the medium for sterile filtration: 20 minutes.
Measure out 180 liters of double distilled or purified tissue culture water at ambient temperature
in a stirred tank. While stirring, add the solutions without heating the water and ensure full recovery of the material from the bottles by rinsing them well.
Add solutions no. 1 and no. 2, then exactly 450 g sodium hydrogen carbonate and wait until complete dissolution.
The recommended L-glutamine concentration is 4mM (116.8 g for 200 liters medium).
Note that higher glutamine levels may lead to excess cytotoxic catabolite formation.
Hektor, InVitrus & TurboDoma media
Add solutions no. 3, no. 4 and no. 5. Adjust end volume with water.
Do not adjust pH (the media are buffered with MOPS)!
InVitrus VP-6 & TurboDoma TP-6 media:
Add solutions no. 3, no. 4, no. 5 and no. 6. Adjust both end volume and, if necessary, pH to 0.1-0.2 units below the desired value with gaseous carbon dioxide.
Sterilize by filtration and store refrigerated at 4-6ºC in the dark.
The filter sterilized medium without L-glutamine expires 9 months after preparation.
Do not store small volumes of culture media in large vessels and protect them from light!